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. 2005 Oct;43(10):5187–5194. doi: 10.1128/JCM.43.10.5187-5194.2005

FIG. 5.

FIG. 5.

Sensitivity and reproducibility of the microarray assay around the limit concentration for mutation detection. Representative results obtained with three diluted multiplex PCR products from two isolates are shown. (A) Asp79Ser80 in MRSA HU2000-62S. (B) Asp79Ser80 in MRSA HU2000-64. Abbreviations for GrlA products: Wt, wild type; DS, Asp79(Wt)Ser80(Wt); DY, Asp79(Wt)Ser80Tyr; DF, Asp79(Wt)Ser80Phe; VS, Asp79ValSer80(Wt); VY, Asp79ValSer80Tyr; VF, Asp79ValSer80Phe. Dilution series of PCR products are 1/10 (□), 1/50(▵), and 1/100 (⋄). The percentages of the fluorescence signal intensities of genotypes related to Asp79Ser80 at 0.5× SSPE were calculated by the following formula: observed signal on probe/highest signal in the same target probes × 100.