TABLE 2.

The 38 probes for PamChip microarray

Probe no.	Sequence (5′→3′)a	Length	Tm (°C)	GC %	Strandb	Mutationc	Gene
1	GCGATATTACCATTACGTGG	20	54	45	S	P451(Wt)	grlB
2	GCGATATTATCATTACGTGG	20	52	40	S	P451S	grlB
3	CCACGTAATGGTAATATCGC	20	54	45	AS	P451(Wt)	grlB
4	CCACGTAATGATAATATCGC	20	52	40	AS	P451S	grlB
5	GTCGTATTTTATACGCAATGTATTC	25	53	32	S	Y47(Wt)	grlA
6	GTCGTATTTTATATGCAATGTATTC	25	52	28	S	Y47Y(Si)	grlA
7	GAATACATTGCGTATAAAATACGAC	25	53	32	AS	Y47(Wt)	grlA
8	GAATACATTGCATATAAAATACGAC	25	52	28	AS	Y47Y(Si)	grlA
9	TCAGTGTACGAAGCAATGGT	20	54	45	S	E84(Wt)	grlA
10	TCAGTGTACAAAGCAATGGT	20	52	40	S	E84K	grlA
11	ACCATTGCTTCGTACACTGA	20	54	45	AS	E84(Wt)	grlA
12	ACCATTGCTTTGTACACTGA	20	52	40	AS	E84K	grlA
13	ACATGGAGACTCCTCAGTGT	20	56	50	S	D79(Wt)S80(Wt)	grlA
14	ACATGGAGACTACTCAGTGT	20	54	45	S	D79(Wt)S80Y	grlA
15	ACATGGAGACTTCTCAGTGT	20	54	45	S	D79(Wt)S80F	grlA
16	ACATGGAGTCTCCTCAGTGT	20	56	50	S	D79VS80(Wt)	grlA
17	ACATGGAGTCTACTCAGTGT	20	54	45	S	D79VS80Y	grlA
18	ACATGGAGTCTTCTCAGTGT	20	54	45	S	D79VS80F	grlA
19	ACACTGAGGAGTCTCCATGT	20	56	50	AS	D79(Wt)S80(Wt)	grlA
20	ACACTGAGTAGTCTCCATGT	20	54	45	AS	D79(Wt)S80Y	grlA
21	ACACTGAGAAGTCTCCATGT	20	54	45	AS	D79(Wt)S80F	grlA
22	ACACTGAGGAGACTCCATGT	20	56	50	AS	D79VS80(Wt)	grlA
23	ACACTGAGTAGACTCCATGT	20	54	45	AS	D79VS80Y	grlA
24	ACACTGAGAAGACTCCATGT	20	54	45	AS	D79VS80F	grlA
25	CTATTTATGAAGCAATGGTACG	22	52	36	S	E88K(Wt)	gyrA
26	CTATTTATAACGCAATGGTACG	22	52	36	S	E88K	gyrA
27	CGTACCATTGCTTCATAAATAG	22	52	36	AS	E88K(Wt)	gyrA
28	CGTACCATTGCGTTATAAATAG	22	52	36	AS	E88K	gyrA
29	CTCATGGTGACTCATCTATTTAT	23	53	34	S	S84(Wt)S85(Wt)	gyrA
30	CTCATGGTGACTCACCTATTTAT	23	55	39	S	S84(Wt)S85P	gyrA
31	CTCATGGTGACTTATCTATTTAT	23	51	30	S	S84LS85(Wt)	gyrA
32	CTCATGGTGACTTACCTATTTAT	23	53	34	S	S84LS85P	gyrA
33	ATAAATAGATGAGTCACCATGAG	23	53	34	AS	S84(Wt)S85(Wt)	gyrA
34	ATAAATAGGTGAGTCACCATGAG	23	55	39	AS	S84(Wt)S85P	gyrA
35	ATAAATAGATAAGTCACCATGAG	23	51	30	AS	S84LS85(Wt)	gyrA
36	ATAAATAGGTAAGTCACCATGAG	23	53	34	AS	S84LS85P	gyrA
37	GTATGGCATGAGTAACGAAGAATATA	26	55	34	S		mecA
38	TATATTCTTCGTTACTCATGCCATAC	26	55	34	AS		mecA

^aDNA sequences of the codons with point mutations are underlined.

^bS, sense strand; AS, antisense strand.

^cD, Asp; E, Glu; F, Phe; K, Lys; L, Leu; P, Pro; S, Ser; V, Val; Y, Tyr; Wt, wild type.