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. 2005 Oct;43(10):5018–5025. doi: 10.1128/JCM.43.10.5018-5025.2005

FIG. 2.

FIG. 2.

PCR-RFLP profiles of cwp66. (A) The amplified 3′ part of the cwp66 gene product was digested with RsaI, SspI, Sca I, and DraI. The different restriction profiles for the enzymes were designated a, b, c, d, e, and f. Lanes M, 100-bp ladders; lanes a, profile a; lanes b, profile b; lanes c, profile c; lanes d, profile d; lanes e, profile e; lanes f, profile f. The digested amplified products were subjected to electrophoresis in a 1.2% (wt/vol) agarose gel. (B) The amplified 5′ part of the cwp66 gene product was digested with DraI, Hind III, KpnI, and RsaI. The different restriction profiles for the enzymes were designated a, b, c, and d. Lanes M, 100-bp ladders; lanes a, profile a; lanes b, profile b; lanes c, profile c; lanes d, profile d. The digested amplified products were subjected to electrophoresis in a 1.2% (wt/vol) agarose gel.