Immunoblot analysis of flagellar preparations for FliC and FliD and cell wall protein extracts for Cwp66 and Cwp84. (A) Flagellar preparation revealed with anti-FliC and anti-FliD rabbit sera. (B) Protein extract revealed with anti-Cwp66 N-terminal rabbit serum (only one band was revealed at the same molecular mass of 50 kDa for all isolates). (C) Protein extract revealed with anti-Cwp66 C-terminal rabbit serum. Several bands at different molecular masses, depending on the strain tested, were revealed. (D) Protein extract revealed with anti-Cwp84 mouse serum. Only one band was revealed at the same molecular mass, 84 kDa, for all isolates. MW, molecular weight low-range standard (Bio-Rad).