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. 2005 Oct;43(10):5158–5163. doi: 10.1128/JCM.43.10.5158-5163.2005

FIG. 2.

FIG. 2.

Detection of multiple mixed HCV genotypes by PSEA-HCV. Five different genotypes (1a, 1b, 2a, 2b, and 3) were mixed in equal amounts and successfully detected by PSEA-HCV. The results from the six separate extension reactions are displayed as separate panels, with each panel indicating which primers were included. All panels show successful amplification with the presence of the IC. The top panel contains predicted peaks for genotypes 1, 2, and 3, whereas panel 4 shows no genotype 4 peak, as expected. The remaining panels show the expected subtyping results of genotypes 1 and 2.