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. 2005 Oct;43(10):5009–5017. doi: 10.1128/JCM.43.10.5009-5017.2005

TABLE 1.

Oligonucleotide primers used for RT-PCR amplification and subsequent nucleotide sequencing

Primer no. Orientation Sequence (5′ to 3′) Nucleotide positiona Target region
1 Sense ACCAGGACCTGGAATACG 2106-2123 P gene
2 Antisense GAGAAAAGCTCATCATCG 2721-2738
3 Sense ACAGGTCAACCAGGTCCA 4873-4890 F gene
4 Sense GCATCGGAATAGCCAGTC 5200-5217
5 Antisense CAGTTTTATGACCAAGTA 5427-5444
6 Sense TCAACAACGAACTCGTCC 5815-5832
7 Antisense GGGCCAAATATTGACAAC 5909-5926
8 Sense CATCTGTAGCCAGAACTCC 6293-6311
9 Sense TATTGCCTCCGATACCTG 6410-6427
10 Antisense GCAGGTATCGGAGGCAAT 6411-6428
11 Sense GTCTCCTCAGTGTTCCTA 6757-6774
12 Antisense AATGTCCGTTGGTAGCGTC 6826-6844
13 Antisense AATGCCGGATCGACCTTA 6857-6874
14 Sense GTCCTTCTCATCCTACTGG 7199-7217 H gene
15 Sense ACTTCCGCGATCTCCACT 7372-7389
16 Antisense AGTGGAGATCGCGGAAGT 7372-7389
17 Antisense ACACTCCGTCTGAGATAGC 7742-7760
18 Sense TCTCAGACGGCGTGTATG 7746-7763
19 Sense TCGACACTCGAGAGATTC 7806-7823
20 Antisense GCATGTCATTCAGCCACC 7851-7868
21 Sense CATCTTATGGGCGGTTGA 8289-8306
22 Antisense GTGAACTGGTCTCCTCTA 8378-8395
23 Antisense TGCCTAAGGCCAATTGAG 8921-8938
24 Antisense CTGTAAGGGATTTCTCAC 8950-8967
a

Nucleotide position based on the genome of strain A75/17 (GenBank accession number AF164967).