TABLE 1.
Primer no. | Orientation | Sequence (5′ to 3′) | Nucleotide positiona | Target region |
---|---|---|---|---|
1 | Sense | ACCAGGACCTGGAATACG | 2106-2123 | P gene |
2 | Antisense | GAGAAAAGCTCATCATCG | 2721-2738 | |
3 | Sense | ACAGGTCAACCAGGTCCA | 4873-4890 | F gene |
4 | Sense | GCATCGGAATAGCCAGTC | 5200-5217 | |
5 | Antisense | CAGTTTTATGACCAAGTA | 5427-5444 | |
6 | Sense | TCAACAACGAACTCGTCC | 5815-5832 | |
7 | Antisense | GGGCCAAATATTGACAAC | 5909-5926 | |
8 | Sense | CATCTGTAGCCAGAACTCC | 6293-6311 | |
9 | Sense | TATTGCCTCCGATACCTG | 6410-6427 | |
10 | Antisense | GCAGGTATCGGAGGCAAT | 6411-6428 | |
11 | Sense | GTCTCCTCAGTGTTCCTA | 6757-6774 | |
12 | Antisense | AATGTCCGTTGGTAGCGTC | 6826-6844 | |
13 | Antisense | AATGCCGGATCGACCTTA | 6857-6874 | |
14 | Sense | GTCCTTCTCATCCTACTGG | 7199-7217 | H gene |
15 | Sense | ACTTCCGCGATCTCCACT | 7372-7389 | |
16 | Antisense | AGTGGAGATCGCGGAAGT | 7372-7389 | |
17 | Antisense | ACACTCCGTCTGAGATAGC | 7742-7760 | |
18 | Sense | TCTCAGACGGCGTGTATG | 7746-7763 | |
19 | Sense | TCGACACTCGAGAGATTC | 7806-7823 | |
20 | Antisense | GCATGTCATTCAGCCACC | 7851-7868 | |
21 | Sense | CATCTTATGGGCGGTTGA | 8289-8306 | |
22 | Antisense | GTGAACTGGTCTCCTCTA | 8378-8395 | |
23 | Antisense | TGCCTAAGGCCAATTGAG | 8921-8938 | |
24 | Antisense | CTGTAAGGGATTTCTCAC | 8950-8967 |
Nucleotide position based on the genome of strain A75/17 (GenBank accession number AF164967).