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. 2025 Sep 30;15:33964. doi: 10.1038/s41598-025-11516-7

Table 4.

Molecular interaction of compounds with DNA gyrase of bacteria binding affinity scores, molecular interaction and bond length.

Compound Substituents Bond Length (Ao) Binding Affinity (k.cal/mol) DNA gyrase binding site (residue)
6a N-methyl piperazine 2.93, 3.20, 3.46, 3.23, 2.93, 3.75 -7.30 ASP 87, ASP 87, SER 116, ALA 117, ASP 87, ARG 91
6b Dimethylamine 2.95, 4.94 -6.90 ARG 91, GLY 114
6c Piperidone 3.50, 4.42 -7.12 SER 116, ARG 91
6d 4-F-Benzylamine 3.08, 3.36, 3.96, 3.56 -7.23 GLN 94, ALA 117, ASP 87, ASP 87
6e Cyclopropylamine 3.62, 3.73 -6.80 ASP 87, ASP 87
6f Indole-3-ethanamine 3.04, 3.11, 2.97, 3.39 -7.57

GLN 94, ALA 117

ASP 87, ASP 87

6 g Thiomorpholine 4.35 -6.64 GLY 114
Streptomycin 2.72, 2.93, 2.81, 3.17, 2.93, 2.97 -7.38 GLY 114, ASP 87, SER 111, ASP 115, PHE 96, GLN 94
Ciprofloxacin 3.49 -5.10 ARG 91
Tetracycline 3.40, 3.09, 2.86 -5.80 PHE 96, GLN 94, ARG 91
Chloramphenicol 3.01, 3.12 -5.73 ALA 117, ARG 91