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. 1998 Jan;36(1):277–280. doi: 10.1128/jcm.36.1.277-280.1998

FIG. 1.

FIG. 1

(a) Agarose gel electrophoresis showing specificity of P4 primers tested with M. pneumoniae and M. genitalium. Lane 1, 100-bp ladder (Pharmacia); lane 2, DNA from M. pneumoniae ATCC 15531 amplified with P4 primers; lane 3, DNA from a clinical isolate positive for M. pneumoniae; lane 4, DNA from a clinical isolate negative for M. pneumoniae; lane 5, DNA from M. genitalium ATCC 33530 amplified with P4 primers; lane 6, 100-bp ladder (Pharmacia). (b) Southern blot of the gel shown in Fig. 1a hybridized with the probe 4A4B specific for M. pneumoniae. Lanes 2 and 3, the probe hybridized to the 345-bp M. pneumoniae-specific fragment only.