Table 2. Dissociation constants, kinetics constants, fluorescence lifetimes of Eu(iii) and corresponding q-values of CCMP variants.
| CCMP variants | K D,Ce(iii) a (μM) | k (M−1 s−1) | τ H2O (μs) | τ D2O (μs) | q |
|---|---|---|---|---|---|
| CCMP | nd | 0.11(1) | 469(7) | 1900(100) | 1.44(5) |
| CCMP-W | 0.32(14) | nd | 392(5) | 1190(70) | 1.55(6) |
| CCMP-C10 | nd | 0.0083(6) | 444(8) | 1800(200) | 1.53(9) |
| CCMP-C16 | nd | nd | 515(3) | 2100(100) | 1.28(4) |
| CCMP-D11E | 0.32(8) | 0.079(6) | 405(3) | 1160(40) | 1.44(4) |
| CCMP-D5N | 5.0(22) | 0.048(2) | 234(2) | 510(20) | 2.23(7) |
| CCMP-P4T | 0.36(11) | 0.0049(5) | 481(5) | 2300(100) | 1.48(3) |
| LBT-C | 0.94(30) | 0.0026(6) | 1274(10) | 2630(70) | 0.11(1) |
a
Dissociation constants were determined by adding the C9W mutation. nd: not determined.