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. Author manuscript; available in PMC: 2025 Oct 2.
Published in final edited form as: Anal Chem. 2015 Nov 18;87(23):11673–11681. doi: 10.1021/acs.analchem.5b02490

Figure 3.

Figure 3.

Optimal conditions for capture and detection of E. histolytica antigen in buffer. Representative fluorescence images and corresponding image analysis of captured “350” antigen, with the antigen capture (0.5–3 min) and detection (1–2 min) performed at different time intervals under ac-EHD induced fluid flow conditions of f = 100 Hz; Vpp = 4 V. Scale bar is 50 μm. Each data point represents the average of three separate measurements (n = 3), and error bars represent standard error of measurements within each experiment.