Figure 4.

Specificity of immunocapture in buffer samples. (a, b) Representative fluorescence images and corresponding image analysis of samples (a) spiked with (1000 pg mL−1) and (b) without “350” antigen in PBS under ac-EHD field strength of f = 100 Hz and Vpp = 4 V. (c,d) Representative fluorescence images and corresponding image analysis of captured “350” antigen (1000 pg mL−1) in PBS on devices without (c) anti-HA antibody and (d) nanoyeast-scFv under ac-EHD field strength of f = 100 Hz and Vpp = 4 V. Each data point represents the average of three separate measurements (n = 3), and error bars represent standard error of measurements within each experiment.