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. Author manuscript; available in PMC: 2025 Oct 2.
Published in final edited form as: Anal Chem. 2015 Nov 18;87(23):11673–11681. doi: 10.1021/acs.analchem.5b02490

Figure 5.

Figure 5.

Rapid capture and detection of E. histolytica antigen. (a) Representative fluorescence images and corresponding fluorescence image analysis of the captured “350 antigen” spiked in PBS at designated concentrations (1 ng mL−1 to 10 fg mL−1) under the applied ac-EHD field strength of f = 100 Hz and Vpp = 4 V. Scale bar is 50 μm. Each data point represents the average of three separate measurements (n = 3), and error bars represent standard error of measurements within each experiment. (b) Representative fluorescence images and corresponding fluorescence image analysis of the captured “350 antigen” spiked in disinfected stool samples at concentrations of 1000 pg mL−1 and 1 pg mL−1 under the applied ac-EHD field strength of f = 100 Hz and Vpp = 4 V. Scale bar is 50 μm. Each data point represents the average of three separate measurements (n = 3), and error bars represent standard error of measurements within each experiment.