Figure 7.
Specificity of immunocapture in disinfected stool samples. (a–c) Representative fluorescence images and (d) corresponding image analysis for the capture and detection of stool samples spiked (a) with (1000 pg mL−1) and (b) without “350” antigen under the applied ac-EHD field strength of f = 100 Hz and Vpp = 4 V. Additional control experiments were performed using stool samples (c) without any spiked antigen under static conditions (without ac-EHD) with simple incubation of target and detection antibody during capture and detection steps, respectively. Scale bar is 50 μm. Each data point represents the average of three separate measurements (n = 3), and error bars represent standard error of measurements within each experiment.