Figure 4.

Overexpressed and endogenous Rabin8 in Hela cells. HeLa cells were transfected over night with empty vector (A and B) or with a construct containing a myc-tagged Rabin8 (C–H). Actin was localized by Alexa488-conjugated phalloidin (A, C, E, and G) and Rabin8 by anti-myc–specific mAb (B, D, F, and H). Protrusions, lamellae, and tails are indicated by arrows. Note that Rabin8-expressing cells contain fewer actin stress fibers and that the cells obtain ruffles, protrusions, and tails. Endogenous Rabin8 (J) and actin (I) were localized by affinity-purified anti-Rabin8 antibodies and Alexa488-conjugated phalloidin, respectively. Rabin8 staining along peripheral actin filaments are indicated by an arrowhead. Bars, 5 μm.