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mMED8 interacts with the Elongin BC complex through a canonical BC-box. 293T cells were transfected with the indicated expression vectors, and immunoprecipitations were performed with the indicated antibodies. Total cell lysates were fractionated on SDS/polyacrylamide gels and analyzed by immunoblotting. WB, immunoblot; IP, immunoprecipitate; LC, light chain. The asterisk denotes a nonspecific background band observed in anti-mMED8 immunoblots of 293T cell lysates.
