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. 2002 Jul 29;99(16):10353–10358. doi: 10.1073/pnas.162424199

Fig 3.

Fig 3.

The mMED8–Elongin BC complex assembles with a Cul2/Rbx1 module. (A and B) Sf21 insect cells were coinfected with the indicated baculoviruses. Immunoprecipitations were performed with the indicated antibodies. Total cell lysates were fractionated by SDS/PAGE and analyzed by immunoblotting. (C) Sf21 cells were coinfected with the indicated baculoviruses. Anti-T7 immunoprecipitations and ubiquitylation assays were performed as described under Materials and Methods. Rxn Mix, reaction mix with Uba1, Ubc5a, GST-ubiquitin, and ATP; GST-Ub, GST-ubiquitin; LC, light chain; WB, immunoblot; IP, immunoprecipitate.