Skip to main content
. 2025 Aug 20;482(16):1197–1208. doi: 10.1042/BCJ20253191

Figure 2. Doxycycline-inducible ASC intrabody expression prevents NLRP3- and AIM2-induced pyroptosis.

Figure 2

(A, B and C) Wildtype parental and the indicated stable intrabody containing iBMDMs were pre-treated overnight with doxycycline (0.1 µg/ml) and cell death kinetics were monitored using IncuCyte imaging: (A) following stimulation with nigericin (5 µM) with or without MCC950 (10 µM) co-treatment; (B) after poly(dA:dT) (1 µg/ml) transfection; and (C) after VbP (2.5 µM) treatment. (D and E) Immunoblot analysis of pyroptotic signalling in total cell lysates and supernatants after (D) nigericin (5 µM) ± MCC950 (10 µM) and (E) poly(dA:dT) treatment (1 and 2 µg/mL). In (B), data are presented as mean ± SEM of pooled data from three independent experiments, P≤0.05 (*), P≤0.001 (***), one-way ANOVA Holm-Šídák’s multiple comparisons test. The IncuCyte data (A and C) symbols represent technical duplicates, and these experiements and the immunoblots (D and E) are representative results of two independent experiments. ASC, apoptosis-associated speck-like protein containing a CARD.