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Dose–response and time course of ectopic lacZ expression. CCS-lacZ hearts from 9.5-dpc embryos were cultured for 48 h with increasing concentrations of the NRG-1 EGF-like domain: (A) none, (B) 2.5 × 10−11 M, (C) 2.5 × 10−10 M, (D) 2.5 × 10−9 M, or (E) 2.5 × 10−8 M, followed by X-Gal staining. CCS-lacZ hearts cultured for 24 h (F) or 48 h (G) in the absence (left heart) or presence of 2.5 × 10−9 M NRG-1 (right heart). [Bar = 0.2 mm (A–G).]
