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. 2025 Jun 30;21(10):1351–1370. doi: 10.1038/s44320-025-00130-4

Reagent and tools table

Reagent/resource Reference or source Identifier or catalogue number
Experimental models
Clostridium sporogenes DSMZ DSM 1664
Escherichia coli Denamur Lab, INSERM IAI1
Klebsiella aerogenes DSMZ DSM 30053
Ruminococcus gnavus DSMZ DSM 108212
Chemicals, enzymes and other reagents
Modified Gifu anaerobic medium (mGAM) Nissui Pharmaceuticals (sold by HyServe, Germany) 1005433-001
Clear untreated sterile 96-well polystyrene plates, u-bottom Corning 3795
Pharmaceutical drug library Prestwick Chemical Libraries Dataset EV2
Pesticide library EMBL Chemical Biology core facility Dataset EV2
Industrial chemicals library Lindell et al, 2024 Dataset EV2
Sweeteners library Blasche et al, 2025 Dataset EV2
Ampicillin Sigma-Aldrich/Merck A9518
Cefixime Cayman Chemical Company 17176
Ceftazidime MedChemExpress HY-B0593/CS-2810
Levofloxacin Cayman Chemical Company 20382
Tetracycline Sigma-Aldrich/Merck 87128
Paroxetine TargetMOI T1636L
Didanosine Cayman Chemical Company 23715
Fenazaflor Sigma-Aldrich/Merck 36504
Acetonitrile VWR 83640.320
Methanol VWR 83638.320
Formic acid Fisher A117-50
Ammonium formate VWR 84884.180
Caffeine Fluka 56396
Amoxicillin Sigma-Aldrich/Merck A8523
Putrescine MetaSci Complete Metabolite Library
Cadaverine Sigma-Aldrich/Merck C8561-1G
Histamine MetaSci Complete Metabolite Library
Tryptamine MetaSci Complete Metabolite Library
2-Phenylethylamine MetaSci Complete Metabolite Library
Software
MassHunter Workstation Agilent v10.1
SkanIt Microplate Reader Software ThermoFischer v 6.1.1
Python v 3.11.5
pandas package McKinney, 2010 v. 2.2.0
scipy package Virtanen et al, 2020 v. 1.11.3
numpy package Harris et al, 2020 v. 1.26.0
scikit-learn package Pedregosa et al, 2012 v. 1.3.2
matplotlib package Hunter, 2007 v. 3.8.0
seaborn package Waskom, 2021 v. 0.13.1
Other
Analytical column: ACQUITY BEH Amide 1.7 μm, 2.1 × 50 mm Waters 186009504
Analytical column: InfinityLab Poroshell 120 EC-C18 1.9 μm, 2.1 × 50 mm Agilent 699675-902
Triple-quadrupole mass spectrometer Agilent 6470
Ultra-high performance liquid chromatography instrument Agilent 1290 Infinity II
Biomek Automated Workstation Beckman Coulter i7
Microplate reader ThermoFisher Multiskan FC, 51119100