Fig. 7.

Phosphorylation of p47-phox in normal neutrophils and neutrophils from diabetic patients with poor glycemic control monitored by Western blotting with a pAb. Neutrophils from healthy individuals (HC; lanes a–d) and diabetes patients with poor glycemic control (DM-PC; lanes e–h) were stimulated with 300 nM PMA for the time periods indicated, and phosphorylation of p47-phox was measured by Western blotting with the pPKC (S) Ab. Neutrophils from the patients and healthy individuals were isolated and stimulated simultaneously. Lanes i and j show the total amount of p47-phox in these subjects monitored with an antibody that recognizes the phosphorylated and nonphosphorylated forms of this protein. Data from four different patients are presented. Conditions for cell stimulation and Western blotting are provided in Materials and Methods.