Table 3.
Genotype Quality (GQ) scores of concordant STR genotypes
| gDNA Input (ng) | GQ1Category, Heterozygotes | |||
| I | II | III | Total | |
| gDNA | 5 | 53 | 580 | 6382 |
| 1 | 43 | 137 | 256 | 4363 |
| 10 | 27 | 140 | 451 | 618 |
| 25 | 26 | 126 | 463 | 615 |
| 50 | 21 | 130 | 474 | 625 |
| 100 | 25 | 110 | 482 | 617 |
| 200 | 78 | 31 | 537 | 646 |
| Total | 225 | 727 | 3243 | 4195 |
| gDNA Input (ng) | GQ Category, Homozygotes | |||
| I | II | III | Total | |
| gDNA | 0 | 9 | 204 | 2134 |
| 1 | 9 | 25 | 168 | 2025 |
| 10 | 2 | 8 | 202 | 212 |
| 25 | 1 | 8 | 199 | 208 |
| 50 | 4 | 11 | 196 | 211 |
| 100 | 8 | 10 | 191 | 209 |
| 200 | 8 | 20 | 184 | 212 |
| Total | 32 | 91 | 1344 | 1467 |
1Category I = GQ scores, originally ≥ 0.25 and <0.40, that have been successfully edited; Category II = GQ scores ≥ 0.40 and ≤ 0.50; Category III = GQ scores ≥ 0.79 and ≤ 0.90. 2GQ score distribution, heterozygote genotypes, gDNA versus all wgaDNA, p < 0.0001. 3GQ score distribution, heterozygote genotypes, wgaDNA produced from 1 ng gDNA input versus other gDNA inputs, p < 0.0001.4GQ score distribution, homozygote genotypes, gDNA versus wgaDNA produced from 1, 100, 200 ng gDNA input, p < 0.0001, 0.01, 0.0001, respectively. 5GQ score distribution, homozygote genotypes, wgaDNA produced from 1 ng gDNA input versus 10, 25, 50, 100 ng gDNA inputs, p = 0.0003, 0.0001, 0.009, 0.02, respectively.