Fig 4.

Phylogeny of the arthropod hemocyanin superfamily. The simplified tree was deduced from the analyses of an amino acid alignment of the Epiperipatus hemocyanin with seven chelicerate, three myriapod, six crustacean, and one insect hemocyanins (Fig. 6). The phenoloxidases (seven sequences) were considered as the outgroup (8). The complete tree may be found in Fig. 7. The numbers at the nodes are support values (from top to bottom): bootstrap numbers estimated by a PAM-based neighbor-joining from the (i) 707- and (ii) 500-aa data sets or estimated by a maximum likelihood quartet puzzling approach (21) with the (iii) 707- and (iv) 500-aa data sets.