Table 1.
The primers used in the experiment.
| Primer name | Primer sequence (5′ → 3′) | Product length (bp) |
|---|---|---|
| sRNA12-F | GCAGGCGTCAAAACAGGT | 344 |
| sRNA12-R | CACTTTGTAAAAAGCATCCTG | |
| qpsRNA12-F | GGGGTGCTTCACTCAACG | 131 |
| qpsRNA12-R | CTCTTATGTTCGCCTTCTGG | |
| 16 s rRNA-F | CAGAAGAAGCACCGGCTAACTC | 87 |
| 16 s rRNA-R | GCGCTTTACGCCCAGTAATT | |
| up-cm-down-F | CTACACAAAATCATTCGGGATGCATAAAAAAACGCCTGCCCGGTTAAGGGAGCGATTGTGTAGGCTGGAG | 1,152 |
| up-cm-down-R | CAGTCTTCATTTGTCGCTATTCGGTGACGAATAATCTATTGATTTATAATTTAACGGCTGACATGGGAATTAG | |
| sRNA12-out-F | CGCAGTAATTCCCTCTTGCC | 542/1250/320 |
| sRNA12-out-R | AACCCCTTATCCCGCACG | |
| bapA-F | AACGACAATACCCCCACTCTG | 199 |
| bapA-R | CTGATTCGCTACTTTCGCCTT | |
| ompC-F | CCGATAACTGGGAAATGGTAGG | 237 |
| ompC-R | CCACGGTGTTGTCTGTATTAGG | |
| sseC-F | TACGAAAGAGGGGGTGAAAG | 188 |
| sseC-R | CACATCTGATAGCCTGTAAGCC | |
| ompD-F | AACCAGGTGAAAGCAGCGAG | 241 |
| ompD-R | GGTAAGCGATGGACGGACG | |
| siiE-F | TTACAAATAGCACCCTGCCAAC | 102 |
| siiE-R | TAGCAACAATGACCTCACCAAG | |
| sseG-F | ACAGTGCTAAGTGGTGGAGGC | 95 |
| sseG-R | TGATGGTAGATAAGACAGGCGAC | |
| prgH-F | CAACACCTGAAAACGCTCCT | 200 |
| prgH-R | CCGAGCAGCCTGAGAAGTT | |
| sseJ-F | TCAATACTTTGGCGGAAGGTTT | 190 |
| sseJ-R | AGAAGGGGTGTAAGATGCGACT | |
| fimW-F | ATTGATCTTACATAAGCGAGCG | 153 |
| fimW-F | AGTGAAAGTAAAGCACCCGTT | |
| rho-F | TAGAACCGTTACCACGCTCC | 206 |
| rho-R | AATCCGCCGTTTCAACCTCC | |
| 16 s rRNA-F | CAGAAGAAGCACCGGCTAACTC | 87 |
| 16 s rRNA-R | GCGCTTTACGCCCAGTAATT |
The underlined part of the primers for the targeting fragment is the primer fragment of the chloramphenicol resistance gene.