Table 1.
Genes up-regulated in STZ-diabetes
| Accession no. | Gene/protein | Relative change in diabetes | Insulin response |
|---|---|---|---|
| Lipid metabolism | |||
| Y14004 | Acyl-CoA thioesterase 1 | 3.71 | +++ |
| U69543 | Hormone-sensitive lipase | 2.38 | ++ |
| AI840013 | Δ3,Δ2-Enoyl-CoA isomerase | 1.93 | +++ |
| AF030343 | Enoyl-CoA hydratase | 1.92 | +++ |
| AV238359 | Carnitine acetyltransferase (similar to) | 1.65 | +++ |
| Z14050 | Dodecenoyl-CoA Δ-isomerase | 1.44 | +++ |
| X85983 | Carnitine acetyltransferase | 1.41 | +++ |
| U07159 | Acetyl-CoA dehydrogenase, medium chain | 1.38 | +++ |
| AW122615 | 47.6-kDa protein B0303.3-like (thiolase family) | 1.35 | +++ |
| AI849271 | 3-Ketoacyl-CoA thiolase (homolog-EST) | 1.33 | +++ |
| Glucose metabolism | |||
| D42083 | Fructose-bisphosphatase 2 | 4.48 | ++ |
| X13586 | 2,3-Bisphosphoglycerate mutase | 1.60 | +++ |
| AW123952 | Lactate dehydrogenase 1, A chain | 1.37 | +++ |
| Others-metabolism | |||
| U48896 | UDP-glucoronosyltransferase 8 | 40.98 | ++ |
| X89998 | 17β-Hydroxysteroid dehydrogenase 4 | 1.75 | ++ |
| AB005450 | Carbonic anhydrase 14 | 1.73 | +++ |
| M33934 | IMP dehydrogenase | 1.28 | + |
| AW046273 | Electron transfer flavoprotein-β-subunit homolog | 1.24 | +++ |
| Transport/trafficking | |||
| U15976 | Fatty acid transporter 1 | 28.18 | +++ |
| X14961 | Fatty acid binding protein 3 | 1.91 | +++ |
| AW125607 | SNAP-α homolog | 1.72 | +++ |
| AF035643 | VAMP-5 (myobrevin) | 1.71 | ++ |
| AF020185 | Dynein, cytoplasmic, light chain 1 | 1.58 | + |
| AI835359 | TRAP-γ signal sequence receptor γ | 1.29 | – |
| U27106 | Adaptor protein complex AP-2, μ 1 | 1.22 | ++ |
| Others | |||
| AV171056 | Ubiquitin-conjugating enzyme E2D2 | 8.26 | +++ |
| AF037454 | Ubiquitin–protein ligase | 1.90 | + |
| AW125420 | Plic-1 homolog | 1.36 | +++ |
| D87898 | ADP-ribosylation factor-1 (ARF1) | 1.27 | + |
Tables 1 and 2 list the up- and down-regulated genes in the STZ-diabetic mice as compared with the controls. The first column lists the GenBank accession no. for the respective gene or EST sequence. The second column lists the name of either the gene or the gene product. The third column lists the relative expression intensity values of the STZ-diabetic as compared to the control. The fourth column lists the insulin response (see Materials and Methods). +++ indicates a >75% response; ++ indicates 50–75% response; + indicates 25–50% response; and – indicates a response of <25%. EST, expressed sequence tag.
Genes that lost their significance when negative intensity values were used without transformation.
Genes that were confirmed with quantitative RT-PCR.