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Nearly complete imprinting of EG cells after differentiation in vivo in mouse chimeras. EG cells were transfected with the pEGFP-N3 vector and injected into blastocysts of C57BL/6 mice, and spleen cells from the chimeras were purified by fluorescence-activated cell sorting. (a) Kvlqt1 analyzed by SSCP-PCR. (b) Snrpn analyzed by SNuPE. (c) Igf2 analyzed by SSCP-PCR; the upper band is a nonspecific product of SSCP as indicated in the parental genomic DNA lanes.
