Figure 3.

Sensorgrams of R06₂₄–mini-TAR complexes. Increasing concentration of R06₂₄, RNA (A) or NP-DNA (B) as indicated by the arrows were injected on a mini-TAR-functionalized sensor chip. Elementary rate constants, k_on and k_off, for bimolecular complex formation were deduced from direct fitting of these plots according to Eqs. 1 and 2 (see Materials and Methods). Experiments were carried out in R buffer (3 mM Mg²⁺) at 23°C.