Figure 6.

Inhibition of Tat-dependent in vitro transcription in HeLa cell nuclear extract. (A) HIV-1 template containing long terminal repeat (LTR) obtained from plasmid p10SLT (34) showing two termination sites (τ and ρ) responsible for the synthesis of two transcripts 325 and 524 nt long. (B) Autoradiograph of transcription products produced from the HIV template in the presence (lane 1) or absence (lane 2) of Tat and of increasing concentration of phosphoramidate aptamers GA (lanes 3–8) and CU (lanes 9–13). The run-off (ρ) and internal artificially terminated (τ) products are marked. (C) Normalized amounts of full-length transcripts as a function of aptamer concentration: NP-DNA(GA) (bold line), NP-DNA(CU) (dotted line).