Table 1.

Activities of TyrRS variants with tyrosine and 3-iodo-l-tyrosine

Enzyme	Amino acid		Enzyme	Amino acid
	Tyrosine	3-Iodo-l-tyrosine		Tyrosine	3-Iodo-l-tyrosine
Wild type (Y37, Q179, Q195)	+++	−	F195	−	−
			L195	++	−
			I195	+	−
F37	+++	−	M195	+	−
H37	+++	+	V195	++	−
A37	++	+++	S195	+++	+
V37	+++	+++	T195	+++	−
I37	++	+++	A195	+++	+
L37	+++	+++	Y195	++	−
M37	+++	+++	H195	+++	−
G37	++	++	N195	++	+
S37	+++	++	K195	−	−
			D195	+	−
A179	++	−	E195	+++	−
E179	−	−	C195	++	+
N179	++	−	W195	−	−
S179	++	−	R195	−	−
Y179	−	−	G195	++	−

The symbols “+++,” “++,” and “+” represent the activities detected with enzyme concentrations of 0.05 μM, 0.5 μM, and 5 μM, respectively, and “−” represents no activity detected with the enzyme, up to a 5 μM concentration.