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Methylation patterns of freshly sorted naïve CD4 cells and developing TH1 and TH2 cells. Naïve CD4 T cells were prepared by sorting for CD44dull/CD62Lbright CD4+ C57BL/6 lymph node T cells. 3DTH1 and 3DTH2 cells were prepared from naïve cells by activation with immobilized anti-CD3 and anti-CD28 under TH1 or TH2 conditions for 3 days; 2rTH2 and 3rTH2 cells activation with soluble anti-CD3, anti-CD28, and antigen-presenting cells, two and three rounds, respectively.
