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Effect of 5′-aza-2′-deoxycytidine treatment on GFP expression
| Treatment | TH1 | 3% TH2 | 30% TH2 |
|---|---|---|---|
| 0 | 0 | 4% | 37% |
| 0.3 μM | 0 | 12% | 32% |
| 2 μM | 0 | 26% | 43% |
Cells were cultured with 5-aza-2′-deoxycytidine during the priming period. They were then washed and maintained in IL-2-containing, drug-free medium for 2 days. Cells were restimulated with phorbol 12-myristate 13-acetate and ionomycin for 24 h, and percent of GFP-expressing cells was measured with a FACScan.
