Figure 2.

(A) Myc-tagged Erg28p (18.4 kDa) was detected by anti-Myc-peroxidase. When Myc is located at the N terminus of Erg28p, a stronger signal was observed than when it was located at the C terminus. Background bands were also observed when using anti-Myc peroxidase. (B) HA-tagged Erg27p (36 kDa) detected by anti-HA-peroxidase. Both N and C terminus-tagged proteins gave the same size bands and signal intensity. (C) HA-tagged Erg26p detected by anti-HA-peroxidase. No band was detected when the HA tag was located at the N terminus. When the HA tag was located at the C terminus, two bands (42 and 40 kDa) were observed.