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Western blot of total protein (lane 1), 10 K pellet (lane 2), 20 K pellet (lane 3), 60 K pellet (lane 4), 130 K pellet (lane 5), and 130 K supernatant (lane 6) probed with anti-Myc to detect Myc-Erg28p (A) and anti-HA to detect Erg27p-HA and Erg26p-HA (B and C, respectively). (D) Kar2p, an ER protein, is also enriched in the 10–60 K membrane pellets.
