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. 2002 Jul 15;99(15):9739–9744. doi: 10.1073/pnas.112202799

Figure 7.

Figure 7

SD2728 (erg27 erg28 double-disrupted strain) transformed with pERG27-HA to form SMO4700, or transformed with pERG27-HA and pMyc-ERG28 to form SMO4701. Sucrose gradient centrifugation of total protein extracts indicates that in SMO4701, the distribution of Erg27p is equally distributed into lighter and heavier ER fraction (D), as indicated by V-ATPase vacuolar marker (A) and ER marker (B). However, in the pERG27-HA single-transformed strain SMO4700, most of the Erg27p (C) is found in the lighter ER or vacuolar fraction using V-ATPase (vacuolar marker, A) and Kar2p (ER marker, B), respectively. Erg28p is always found in the heavier ER fraction (E).