Figure 8.
Copurification of the C-4 demethylation complex using anti-HA matrix beads. Solubilized membrane protein of SMO4701 (lane 1) and SMO5001 (lane 2) were coimmunoprecipitated with mouse anti-HA matrix beads. Four protein bands, 18.4, 35, 36, and 40 kD, were identified by immunoassay as Erg28p, Erg25p, Erg27p, and Erg26p, respectively.