Table 1.

Primers used for PCR cloning and epitope tagging of ERG26, ERG27, and ERG28 genes

Genes	Designed primers (5′-3′)
ERG26	ERG26-F: ccGAATTCatgtcaaagatagattcagtttt
	ERG26-F-HA: ccGAATTCATGTACCCATACGATGTTCCAGATTACGCTatgtcaaagatagattcagtttt
	ERG26-R: gcGTCGACttacaaaccttcgtccatcc
	ERG26-R-HA: cgcGTCGACTTAAGCGTAATCTGGAACATCGTATGGGTAcaaaccttcgtccatccag
ERG27	ERG27-F: ccGAATTCatgaacaggaaagtagctatc
	ERG27-F-HA: ccGAATTCATGTACCCATACGATGTTCCAGATTACGCTatgaacaggaaagtagctatc
	ERG27-R: gcGTCGACttaaatgggggttctagtttca
	ERG27-R-HA: cgcGTCGACTTAAGCGTAATCTGGAACATCGTATGGGTAaatgggggttctagtttcaac
ERG28	ERG28-F: ccGGATCCatgttcagcctacaagacgtaata
	ERG28-F-Myc: ccGGATCCATGTCTGAACAAAAATTGATTTCTGAAGAAGATTTGatgttcagcctacaagacgtaata
	ERG28-R: cgcATCGATttaccaagcaacaccagtgtagt
	ERG28-R-Myc: cgcATCGATTTACAAATCTTCTTCAGAAATCAATTTTTGTTCAGAccaagcaacaccagtgtagtatt
	ERG28-F: ccGAATTCatgttcagcctacaagacgtaata

F and R indicate forward and reverse primers directly. Underlined sequences represent engineered restriction sites. Nucleotides in capitals represent epitope-coding sequences.