Table 1.
List of tryptic fragments of glutathionylated rhTrx
| Experimental m/z | Calculated m/z | Tryptic fragment |
|---|---|---|
| 1148.7 | 1148.5 | 72–80 |
| 1165.7 | 1165.6 | 85–95 |
| 1221.8 | 1221.6 | 82–93 |
| 1258.8 | 1258.7 | 94–104 |
| 1292.6 | 1292.6 | 72–81* |
| 1336.8 | 1336.6 | 8–20 |
| 1453.7 | 1453.5 | 72–80† |
| 1463.9 | 1463.8 | 36–47 |
| 1479.9 | 1479.8 | 36–47* |
| 1581.8 | 1581.6 | 72–81‡ |
| 1606.9 | 1606.9 | 81–95 |
| 2148.1 | 2148.1 | 85–104 |
| 2149.3 | 2149.1 | 1–20 |
| 2942.6 | 2942.4 | 8–35 |
rhTrx was treated with GSSG 5 mM for 2 h at room temperature and subjected to tryptic digestion; the fragments were analyzed by MALDI-TOF. Glutathionylated fragments are shown in italics.
*
Methionine oxidized to sulfoxide (MSO).
†
Fragment 1148.7 + one GSH moiety (305 kDa).
‡
Fragment 1276.6 and + one GSH moiety.