Figure 3.

Schematic diagram showing the pattern of NH-NH and CαH-CαH NOEs predicted for conformations of M_Hβ₂m (a) lacking and (b) containing the β bulge involving residues 53 and 54. CαH-CαH region (c) and NH-NH region (d) of a ¹H-¹H NOESY spectrum of M_Hβ₂m acquired at pH 5.7, 37°C, in D₂O and H₂O, respectively. Crosspeaks consistent with the presence of the β bulge in solution are marked in bold and underlined. Crosspeaks expected were M_Hβ₂m to adopt a conformation in solution identical to that in a are shown as solid circles.