Figure 4.

The nanomechanical response after hybridization to targets with different overhanging extensions. Sequential experiments were performed on a single cantilever followed by dehybridization washing steps, and differential measurements were extracted. The oligonucleotide B2C is the perfect matching complement, whereas 5′-(A)₁₂-BioB2C (Table 1) has an overhanging single-stranded DNA end toward the lumen dangling away from the cantilever surface, and 5′-(A)₆-BioB2C-(A)₆-3′ has overhanging ends on both sides of the immobilized probe on the cantilever surface.