Fig. 5. PREPi alleviates steatohepatitis in a High-Fat Diet (HFD) mouse model. (A) Experimental design and treatment timeline for establishing the steatohepatitis model in C57BL/6 mice. (B) Representative liver images from control, model (HFD-fed), and PREPi-treated groups. (C) Hematoxylin and Eosin (H & E) staining of liver tissues along with quantification of liver histology using the NAFLD Activity Score (NAS) in control, model, and PREPi-treated groups. (D) Mice body weight progression curve across all three treatment groups over 24 weeks. (E) Glucose Tolerance Test (GTT) results in control, model, and PREPi-treated groups. (F) Pyruvate Tolerance Test (PTT) results in control, model, and PREPi-treated groups. (G–H) ELISA-based analysis of serum liver enzyme levels (ALT and AST) in control, model, and PREPi-treated groups. (I–L) Lipid profile analysis, including Total Cholesterol (TC), Alkaline Phosphatase (ALP), Low-Density Lipoprotein (LDL), and High-Density Lipoprotein (HDL) in control, model, and PREPi-treated groups. All data are presented as means ± SD. Statistical significance is indicated as follows: p < 0.05 (*), p < 0.01 (**), p < 0.001 (***), p < 0.0001 (****), “ns” denotes non-significant differences.