Table 3.

Effect of antisense MO on the ventralizing activity of Bmps. Capped mRNA and glce MOs were injected into the yolk of one- to two-cell embryos as in the experiments of table 1. The resulting phenotypes were scored at 48 hpf. For the rescue experiments, embryos were first injected with bmp2b or bmp4 mRNA and after randomization half received the indicated amount of MO before reaching the 4-cell stage. Phenotype severity was scored as in Table 1.

Treatments	Strength of Ventralization
	No.	Wild	V1	V2	V3	V4
		(%)	(%)	(%)	(%)	(%)
Uninjected	40	100	0	0	0	0
bmp2b mRNA (50 pg)	62	0	0	6	18	76
bmp2b mRNA (50 pg) glce-A MO (8 ng) glce-B MO (8 ng)	60	15	15	31	25	16
bmp4 mRNA (100 pg)	60	0	0	0	15	85
bmp4 mRNA (100 pg) glce-A MO (8 ng) glce-B MO (8 ng)	60	21	18	23	23	15