Fig. 1.

DLC2 isoforms. (A) Functional domains of the DLC2 isoforms. DLC2α and DLC2β each contains a SAM, a RhoGAP, and a START domain, but they differ in their N-terminal sequence. The difference in the amino acid sequence was located at the first 60 aa in DLC2α and the first 52 aa in DLC2β. DLC2γ contains a RhoGAP and a START domain. DLC2δ only contains a SAM domain. (B) Effects on actin cytoskeleton in Swiss 3T3 transfected with DLC2α, DLC2β, DLC2γ, and EGFP vector. After transfection, the cells were starved in DMEM for 24 h and then stimulated with LPA for 15 min. Stress fibers were visualized with tetramethylrhodamine B isothiocyanate-labeled phalloidin, and the nuclei were stained by DAPI.