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. 2005 Oct 10;102(42):15207–15212. doi: 10.1073/pnas.0504501102

Fig. 5.

Fig. 5.

DLC2 reduced the migration rates of HepG2 cells. (A) Cell migration of DLC2γ stable cell lines in in vitro scratch wound healing assay. HepG2-γ-1, HepG2-γ-2, HepG2-γ-4, and HepG2-EGFP-V were treated with Mitomycin C before wounding on day 0. Photographs were taken at day 1 and 2, respectively, after the wound was made. (B) Transwell migration assay of DLC2γ stable cell lines. Transwell filter was used to measure the migration ability of HepG2-γ-1, HepG2-γ-2, HepG2-γ-4, and HepG2-EGFP-V. Migrated cells at the lower surface of the transwell filter were stained and counted. (C) The numbers of the migrated cells in HepG2-γ-1 (*, P < 0.0001, t test), HepG2-γ-2 (*, P = 0.0001, t test), and HepG2-γ-4 (*, P < 0.0001, t test) were significantly lower than that of the mock-transfected HepG2-EGFP-V cells.