Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2002 Jul 29;99(16):10753–10758. doi: 10.1073/pnas.172117099

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2002, The National Academy of Sciences

PMC Copyright notice

Fig 1. — (A) Isolation of recombinant progeny for genetic mapping and virulence linkage analysis. Clonal isolates of the type I strain GT-1 resistant to 5-fluoro-2-deoxyuridine (FUDR^R) and type III strain CTG resistant to adenine arabinoside and sinefungin (AraA^R/SNF^R) were crossed, and recombinant progeny clones were selected with drug selections (AraA^R/FUDR^R or SNF^R/FUDR^R combination). Alternatively, progeny clones were chosen at random and genotyped to obtain recombinant ones. (B) An example of genotyping using PCR/RFLP analysis for the SAG3 gene amplified from the parental strains (indicated as I, III) and the progeny (indicated as 1–9). PCR products were digested with restriction enzyme NciI and resolved in 3% agarose gel in the presence of ethidium bromide. DNA marker = φ174 DNA digested with HaeIII.