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SDS/PAGE and immunoblot analyses of rHBHA, chemically methylated rHBHA and nHBHA. Purified rHBHA (A), purified rHBHA subjected to chemical lysine methylation for 1, 5, and 15 min (B, C, and D, respectively), and purified nHBHA (E) were analyzed by SDS/PAGE followed by Coomassie blue staining (Left) or by immunoblotting using mAb 4057D2 (Right). The sizes of the Mr markers given in kDa are shown in the left margin.
