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. 2002 Jul 29;99(16):10759–10764. doi: 10.1073/pnas.162246899

Fig 7.

Fig 7.

SDS/PAGE, autoradiography, and immunoblot analyses of rHBHA incubated with bacillus Calmette–Guérin lysate in the presence of radiolabeled AdoMet. Clarified whole-cell lysate was incubated for different times with radiolabeled AdoMet in the absence (A) or in the presence of rHBHA (C–F). The incubation times tested were 3 hr (A, C, and F), 1 hr (E), and 10 min (D). In C, the WCL was boiled for 5 min before incubation with rHBHA. B shows rHBHA incubated with radiolabeled AdoMet in the absence of WCL. Left shows the polyacrylamide gel stained with Coomassie blue, Center shows the autoradiography, and Right shows the immunoblot by using mAb 4057D2. The sizes of the Mr markers given in kDa are shown in the left margin.