Table 2.
List of primary and secondary antibodies used for immunofluorescence assays.
| Inmmunogen | Reference | Company | Isotype | Dilution | Type |
|---|---|---|---|---|---|
| CK15 | sc-47697 | Santa Cruz | IgG2a | 1:50 | Primary |
| CK3/K76 | CBL218 | Millipore | IgG1 | 1:50 | Primary |
| Integrin β4 | ab29042 | Abcam | IgG1 | 1:40 | Primary |
| Ki67 | MAB4190 | Millipore | IgG1 | 1:40 | Primary |
| ZO-1 | ab190085 | Abcam | IgG | 1:40 | Primary |
| α-SMA | ab7817 | Abcam | IgG2a | 1:400 | Primary |
| anti-Goat IgG | A11057 | ThermoFisher scientific | IgG | 1:1,000 | Secondary |
| anti-Rabbit IgG | ab175471 | Abcam | IgG | 1:1,000 | Secondary |
| anti-Rabbit IgG | A11070 | ThermoFisher scientific | IgG | 1:1,000 | Secondary |
| anti-Mouse IgG1 | A21121 | ThermoFisher scientific | IgG | 1:1,000 | Secondary |
| anti-Mouse IgG1 | A21124 | ThermoFisher scientific | IgG | 1:1,000 | Secondary |
| anti-Mouse IgG2a | A21131 | ThermoFisher scientific | IgG | 1:1,000 | Secondary |
| anti-Mouse IgG2a | A21134 | ThermoFisher scientific | IgG | 1:1,000 | Secondary |