Fig 2.

Oxidative stress tolerance by preexposure to sublethal oxidative stress. (A) Leaf discs, pretreated for 17 hr with water (gray bar) or 0.1 μM MV (black bar), were exposed to a 1-μM MV solution. At regular intervals, ion leakage was measured as conductivity of the solution. Values are averages of nine independent experiments. (B) Expression of antioxidant genes, glutathione peroxidase (GPx) and cytosolic Cu/ZnSOD (SodCc), during the treatment. Total RNA was extracted from six leaf discs sampled in two independent experiments and subjected to RNA gel blot analysis. The same membrane was used for hybridization with both genes.