Table 2.
Differentially ESTs
| Accession no. | Clone | Length, bp | Gene name (tentative) | Homologue | E value | Function (putative) | Ratio |
|---|---|---|---|---|---|---|---|
| Stress defense | |||||||
| AJ344612 | t18-2-5 | 448 | PRB1-b | Basic PRB-1b (N. tabacum) emb|X66942 | 0A | Antimicrobial protein | 19.9 |
| AJ344611 | t12-2-1 | 376 | Chitinase4 | Chitinase class 4 (Vigna unguiculata) emb|CAA61281 | 2e-07 | Antimicrobial protein | 7.3 |
| AJ344578 | c15-1-4 | 517 | CBP20 | Pathogen- and wound-inducible antifungal protein CBP20 (N. tabacum) gb|S72452 | e-159A | Antimicrobial protein | 6.5 |
| Terpenoid biosynthesis | |||||||
| AJ344602 | g2-1-2 | 228 | EAS | 5-epi-aristoiochene synthase str319 gene (N. tabacum) emb|Y08847 | 9e-43A | Phytoalexins synthesis | 18.0 |
| AJ344595 | g14-2-4 | 382 | VS | Vetispiradiene synthase (S. tuberosum) gb|AAD02223 | 5e-31 | Phytoalexins synthesis | 13.4 |
| AJ344620 | t7-5-4 | 332 | MVD | Putative mevalonate diphosphate decarboxylase (Arabidopsis thaliana) gb|AAC67348 | 4e-10AB | Terpenoid biosynthesis | 7.3 |
| AJ344605 | g6-3-7 | 397 | ATPCL | ATP citrate lyase (Capsicum annuum) gb|AAK13318 | 9e-22 | Cytosolic acetyl-CoA synthesis substrate | 13.6 |
| Phenylpropanoid biosynthesis | |||||||
| AJ344572 | a9-3-4 | 280 | ISI10a | Immediate-early salicylate-induced glucosyltransferase (IS10a) (N. tabacum) gb|U32643 | e-147A | Phenylpropanoid glucosyltransferase | 5.0 |
| AJ344574 | c11-3-3 | 200 | CCoAOMT | Caffeoyl-CoA O-methyltransferase (N. tabacum) emb|Z56282 | 2e-52A | Lignins/lignans biosynthesis | 2.5 |
| AJ344604 | g6-2-13 | 526 | LDOX | Leucoanthocyanidin dioxygenase 2, putative (A. thaliana) gb|AAG21532 | 9e-40 | Anthycyanidins biosynthesis | 2.3 |
| Cellular protection/detoxification | |||||||
| AJ344607 | g9-2-2 | 505 | MDR | P-glycoprotein/multidrug resistance-like protein (A. thaliana) emb|CAB71875 | 2e-40 | Transporter | 21.4 |
| AJ344618 | t7-4-7 | 420 | GST | Glutathione S-transferase GST 12 (Glycine max) gb|AAG34802 | 2e-10 | Detoxification | 5.9 |
| AJ344571 | a9-1-2 | 368 | EH-1 | Epoxide hydrolase (N. tabacum) gb|U57350 | 0A | Detoxification | 4.8 |
| AJ344584 | c18-1-2 | 409 | DNAJ | DNAJ protein-like (A. thaliana) emb|CAB86070 | 7e-33 | Chaperone | 4.1 |
| Energy/carbohydrate metabolism | |||||||
| AJ344613 | t18-3-7 | 389 | PI-transporter | Mitochondrial phosphate transporter (G. max) dbj|BAA31582 | 3e-16 | Mitochondrial transporter-ATP synthesis | 12.5 |
| AJ344586 | c2-1-10 | 408 | UCP | Putative uncoupling protein 3, mitochondrial (Mus musculus) ref|NP_033490 | 2e-24AB | Protons transporter, energy dissipation | 2.3 |
| AJ344614 | t18-4-18 | 306 | AGP | ADP-glucose pyrophosphorylase small subunit (S. tuberosum) emb|CAA43489 | 5e-04 | Chloroplast starch biosynthesis | 0.5 |
| AJ344615 | t2-1-3 | 397 | Ycf3 | Chloroplast genome, Ycf3 protein (N. tabacum) refNC_001879 | e-98A | Assembly of photosystem I subunits | 0.5 |
| Signal transduction | |||||||
| AJ344573 | a9-7/10 | 220 | Lox1 | Lipoxygenase Lox1 (N. tabacum) emb|X84040 | e-110A | Oxylipin metabolism | 4.5 |
| AJ344609 | g9-6-1 | 269 | Lox2 | Lipoxygenase (S. tuberosum) gb|U24232 | 5e-20 | Oxylipin metabolism | 3.7 |
| AJ344617 | t7-1-14 | 506 | MFP | Multifunctional protein of glyoxysomal fatty acid β-oxidation (Brassica napus) emb|AJ000886 | 5e-13 | Oxylipin metabolism | 3.1 |
| AJ344610 | t12-1-7 | 396 | TPK | Serine/threonine/tyrosine-specific protein kinase APK1A (A. thaliana) dbj|BAA02092 | 6e-06 | Signal transduction | 7.2§ |
| AJ344596 | g14-3-4 | 289 | HRA | Hypersensitive reaction-associated Ca2+-binding protein (Phaseolus vulgaris) gb|AAD47213 | 6e-04 | Signal transduction | 3.9§ |
| AJ344616 | t7-1-12 | 550 | CIPK | Putative CBL-interacting serine/threonine kinase (A. thaliana) gb|AAG50566 | 5e-18 | Signal transduction | 2.1 |
| AJ344598 | g17-2-13 | 553 | WRKY11 | WRKY DNA-binding protein (S. tuberosum) emb|CAB97004 | 8e-44 | Transcription factor | 7.2 |
| AJ344590 | c20-1-4 | 361 | pabf | DNA-binding protein (pabf) (N. tabacum) gb|U06712 | 0A | Transcription factor | 0.5§ |
| Protein synthesis | |||||||
| AJ344575 | c14-3-4 | 333 | 60S-L23A | 60S ribosomal protein L23A (L25) (N. tabacum) gb|L18908 | e-171A | Protein synthesis | 2.6 |
| Other/unclassified | |||||||
| AJ344580 | c15-2-8 | 445 | Putative protein (A. thaliana) emb|CAB88533 | 1e-04 | 4.8 | ||
| AJ344594 | g10-1-1 | 384 | Putative ABA-responsive gene (A. thaliana) dbj|BAB11190 | 3e-10 | 4.5 | ||
| AJ344581 | c15-3-4 | 471 | Putative protein (A. thaliana) gb|AAF63779 | 9e-18 | 2.8 | ||
| AJ344603 | g20-2-20 | 337 | Putative protein (A. thaliana) gb|AAF14679 | 3e-18 | 2.4 | ||
*
Cloned cDNAs fragments without homology are listed here; induced: a1-1-7 (AJ344568), a14-1-4 (AJ344569), a19-3-4 (AJ344570), c14-7-4 (AJ344576), c15-1-2 (AJ344577), c15-11-4 (AJ344579), c19-3-10 (AJ344585), c2-4-1 (AJ344588), c2-9-14 (AJ344589), c3-2-4 (AJ344591), g15-4-1 (AJ344597), g18-5-1 (AJ344599), g18-7-5 (AJ344601), g6-4-4 (AJ344606), g9-2-6 (AJ344608), t7-4-8 (AJ344619); repressed: c15-7-1 (AJ344582), c15-8-5 (AJ344583), c2-11-14 (AJ344587), c4-1-2 (AJ344592), c4-3-3 (AJ344593), g18-6-5 (AJ344600).
†
All are BLASTX, except A = BLASTN; B = search result with Solanacae ESTs as a query sequence (see Materials and Methods for details).
‡
Ratios between the values from 0.1 μM MV and water pretreated samples identified by cDNA array analysis.
§ Ratios where one of the values was less than background plus 2 times SD.