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. 2002 Jul 19;99(16):10881–10886. doi: 10.1073/pnas.152330299

Fig 2.

Fig 2.

Histological GUS staining of cross-sections of rice plantlets. The older leaf sheaths enclose the blade of the youngest leaf. (A) Line R1, hemizygous (T2 generation): normal GUS expression of HRintG in vascular bundles and epidermal cells of the leaf blade and in vascular bundles and parenchyma cells of the leaf sheaths. (B) Line R1, homozygous (T2): silenced expression in the vascular bundles, expression exclusively in epidermal cells. (C) Line HRintG-100 containing a GUS gene under control of a deletion variant of the RTBV promoter, which is only active in the epidermis. (D) Line R2, homozygous (T3): expression exclusively in the epidermal cells. (E) Line R1, homozygous (T3): almost completely silenced expression. (F) F1 generation plant of a sexual cross between homozygous R2 and a nontransgenic plant. (G) F2 generation plant (hemizygous) of the sexual cross in F. (H) Line R1, homozygous (T2): restored expression in the vascular bundles after germination on 30 μM 5-AC.