Fig 6.

Analysis of transgene-derived RNAs by RNase protection. (A) Schematic presentation of the expected protection pattern. dps, downstream promoter sequence; SD, splice donor site; SA, splice acceptor site; 5′, protected 5′ exon of the spliced RNA; 3′, protected 3′ exon of the spliced RNA. The region covered by the antisense probe used for the assay in B is shown schematically. Length of expected fragments is indicated. (B) RNase protection assay with total RNA isolated from the leaves of freshly germinated seedlings of untransformed and R1 rice plants; fragments are designated as in A. 5-AC, seedlings grown in the absence (−) or presence (+) of 30 μM 5-AC; MWM, molecular weight marker. (C) Quantification of RNA fragments from B and correlation to the respective GUS activity of the plants. Values are relative to the normally expressing hemizygous line R1 (= 100), and the stimulation factor for incubation with 30 μM 5-AC is given.